| Field | Specification |
|---|---|
| Target | |
| Alternative names | SWI/SNF complex subunit SMARCC1; BRG1-associated factor 155; BAF155; SWI/SNF complex 155 kDa subunit; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1; SMARCC1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SMARCC1/BAF155 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18S70 is a rabbit monoclonal antibody that recognizes SWI/SNF complex subunit SMARCC1 in human and rat samples and is validated for Western blot, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 122.9 kDa, and the supplier reports an observed band at about 155 kDa.
BAF155 (SMARCC1) is a core scaffold subunit of SWI/SNF (BAF) chromatin remodeling complexes. It helps assemble and stabilize the complex and may stimulate the activity of its ATPase subunit, so it influences gene regulation during development and differentiation.
| Target | SWI/SNF complex subunit SMARCC1 (gene SMARCC1; UniProt Q92922, human) |
|---|---|
| Clone | 18S70 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 122.9 kDa |
| Observed MW | 155 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human SMARCC1/BAF155.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places SWI/SNF complex subunit SMARCC1 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
