| Field | Specification |
|---|---|
| Target | |
| Alternative names | E3 ubiquitin-protein ligase SMURF2; hSMURF2; HECT-type E3 ubiquitin transferase SMURF2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2; SMURF2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SMURF 2/SMURF2 recombinant protein (Position: W303-Q387). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects E3 ubiquitin-protein ligase SMURF2 in human, mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 86.2 kDa, and the supplier reports an observed band at about 86 kDa.
SMURF2 is a HECT-type E3 ubiquitin ligase that dampens TGF-β signaling. Working with SMAD7, it ubiquitinates the TGF-β receptor for degradation, and it can also target receptor-regulated SMADs and other substrates, so it is studied in fibrosis, cancer and cellular senescence.
| Target | E3 ubiquitin-protein ligase SMURF2 (gene SMURF2; UniProt Q9HAU4, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 86.2 kDa |
| Observed MW | 86 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HCT116 cells, human U2OS cells, human SH-SY5Y cells, rat testis tissue, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Recombinant human SMURF 2/SMURF2 fragment (Trp303–Gln387), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places E3 ubiquitin-protein ligase SMURF2 in the nucleus, cytoplasm, cell membrane and membrane raft. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
