| Field | Specification |
|---|---|
| Target | |
| Alternative names | Zinc finger protein SNAI1; Protein snail homolog 1; Protein sna; SNAI1; SNAH |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SNAIL/SNAI1 recombinant protein (Position: M1-K170). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects zinc finger protein SNAI1 in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 29.1 kDa, and the supplier reports an observed band at about 29 kDa.
Snail (SNAI1) is a zinc finger transcriptional repressor that is a master inducer of epithelial–mesenchymal transition (EMT). It binds E-box sequences in the E-cadherin (CDH1) promoter and recruits corepressors such as LSD1 to silence it, so it is widely studied in development, fibrosis and cancer invasion.
| Target | Zinc finger protein SNAI1 (gene SNAI1; UniProt O95863, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 29.1 kDa |
| Observed MW | 29 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A431 cells, human HepG2 cells, human A549 cells, human PC-3 cells, human K562 cells, human SW620 cells, human Raji cells, rat RH35 cells, mouse liver tissue, mouse lung tissue, mouse heart tissue, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): 293T cells.
Immunogen
Recombinant human SNAIL/SNAI1 fragment (Met1–Lys170), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places zinc finger protein SNAI1 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed in a variety of tissues with the highest expression in kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhu Xiao-Ren et al. (2025) A novel identified epithelial ligand-receptor-associated gene signature highlights POPDC3 as a potential therapy target for non-small cell lung cancer. Cell Death & Disease. 10.1038/s41419-025-07410-9
Jingwei Peng et al. (2026) CBLC-mediated ubiquitination stabilizes METTL1, enhances N7-methylguanosine modification of ESRRA, and promotes the progression of endometrial cancer. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2026.152809
Yongli Li et al. (2019) p53-Mediated PI3K/AKT/mTOR Pathway Played a Role in PtoxDpt-Induced EMT Inhibition in Liver Cancer Cell Lines. Oxidative Medicine and Cellular Longevity. 10.1155/2019/2531493
