| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Suppressor of cytokine signaling 2;SOCS-2;Cytokine-inducible SH2 protein 2;CIS-2;STAT-induced STAT inhibitor 2;SSI-2;SOCS2;CIS2, SSI2, STATI2; |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human SOCS2 recombinant protein (Position: R96-V198). Human SOCS2 shares 96.1% and 95.1% amino acid (aa) sequence identity with mouse and rat SOCS2, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of SOCS2 (Suppressor of cytokine signaling 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-SOCS2 Antibody Picoband® catalog # PB9400. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human SOCS2 recombinant protein (Position: R96-V198). Human SOCS2 shares 96.1% and 95.1% amino acid (aa) sequence identity with mouse and rat SOCS2, respectively. (reported region: R96-V198).
- Molecular weight context: reported MW: 22 kDa; calculated MW: 22172 MW
- Reactivity: Human,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Suppressor of cytokine signaling 2; Suppressor of cytokine signaling 2. Suppressor of cytokine signaling 2 is a protein that in humans is encoded by the SOCS2 gene. This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signalling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including erythropoietin, GM-CSF, IL10 and interferon-gamma (IFN-gamma). The protein encoded by this gene is found to interact with the cytoplasmic domain of insulin-like growth factor 1 receptor (IGF1R), and thus is thought to be involved in the regulation of IGF1R mediated cell signaling. By cytogenetic and radiation hybrid mapping, Yandava et al. (1999) mapped the SOCS2 gene to chromosome 12q21.3-q23. Functional note: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin- protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Expression/tissue context: High expression in heart, placenta, lung, kidney and prostate.
Research relevance and current trends
- Cytokines: Researchers commonly examine how SOCS2 (Suppressor of cytokine signaling 2) relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how SOCS2 (Suppressor of cytokine signaling 2) relates to this theme using model systems and orthogonal readouts.
- Innate Immunity: Researchers commonly examine how SOCS2 (Suppressor of cytokine signaling 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative SOCS2 (Suppressor of cytokine signaling 2) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Family / similarity context: Contains 1 SH2 domain.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
