| Field | Specification |
|---|---|
| Target | |
| Alternative names | Suppressor of cytokine signaling 2;SOCS-2;Cytokine-inducible SH2 protein 2;CIS-2;STAT-induced STAT inhibitor 2;SSI-2;SOCS2;CIS2, SSI2, STATI2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SOCS2 recombinant protein (Position: R96-V198). Human SOCS2 shares 96.1% and 95.1% amino acid (aa) sequence identity with mouse and rat SOCS2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects suppressor of cytokine signaling 2 (gene SOCS2) in human and rat samples and is validated for Western blot. The predicted molecular weight is 22.2 kDa, and the supplier reports an observed band at about 22 kDa.
Suppressor of cytokine signaling 2 (SOCS2) is an SH2 domain protein that serves as the substrate-recognition subunit of a cullin-5 E3 ubiquitin ligase. It recognizes phosphorylated receptors such as the growth hormone receptor and the erythropoietin receptor and marks them for proteasomal degradation, making it an important feedback brake on growth hormone signaling and body growth.
| Target | Suppressor of cytokine signaling 2 (gene SOCS2; UniProt O14508, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 22.2 kDa |
| Observed MW | 22 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|
Samples with a confirmed band (WB): PC-12 cells, SW620 cells, HeLa cells, MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Recombinant human SOCS2 fragment (Arg96–Val198), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 96.1% and 95.1%, respectively.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places suppressor of cytokine signaling 2 in the cytoplasm. Tissue expression noted by UniProt (human): High expression in heart, placenta, lung, kidney and prostate. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
