| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Suppressor of cytokine signaling 4;SOCS-4;Suppressor of cytokine signaling 7;SOCS-7;SOCS4;SOCS7; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human SOCS4 recombinant protein (Position: M291-C440). Human SOCS4 shares 98% amino acid (aa) sequence identity with mouse SOCS4. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of SOCS4 (Suppressor of cytokine signaling 4) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-SOCS4 Antibody Picoband® catalog # PB9506. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human SOCS4 recombinant protein (Position: M291-C440). Human SOCS4 shares 98% amino acid (aa) sequence identity with mouse SOCS4. (reported region: M291-C440).
- Molecular weight context: reported MW: 51 kDa; calculated MW: 50623 MW
- Reactivity: Human
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Suppressor of cytokine signaling 4; Suppressor of cytokine signaling 4. SOCS4, also known as Suppressor of cytokine signaling 4, is mapped to 14q22.1. The protein encoded by this gene contains a SH2 domain and a SOCS BOX domain. The protein thus belongs to the suppressor of cytokine signaling (SOCS), also known as STAT-induced STAT inhibitor (SSI), protein family. SOCS family members are known to be cytokine-inducible negative regulators of cytokine signaling. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. Functional note: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. Substrate-recognition component of a SCF-like ECS (Elongin BC- CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Inhibits EGF signaling by mediating the degradation of the Tyr-phosphorylated EGF receptor/EGFR. . Reported localization: Membrane; Multi-pass membrane protein. Expression/tissue context: Placenta, liver, and to a much smaller extent, in lung.
Research relevance and current trends
- Adapters: Researchers commonly examine how SOCS4 (Suppressor of cytokine signaling 4) relates to this theme using model systems and orthogonal readouts.
- Signal Transduction: Researchers commonly examine how SOCS4 (Suppressor of cytokine signaling 4) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative SOCS4 (Suppressor of cytokine signaling 4) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Contains 1 SH2 domain.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
