| Field | Specification |
|---|---|
| Target | |
| Alternative names | Superoxide dismutase [Mn], mitochondrial; SOD2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human SOD2, different from the related mouse sequence by one amino acid, and from the related rat sequence by four amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 2B12B1 is a mouse monoclonal antibody that recognizes superoxide dismutase (gene SOD2) in human and mouse samples and is validated for Western blot and IHC. The predicted molecular weight is 24.8 kDa, and the supplier reports an observed band at about 25 kDa.
Superoxide dismutase 2 (SOD2, MnSOD) is a manganese-containing enzyme in the mitochondrial matrix that converts superoxide made during respiration into hydrogen peroxide and oxygen. As a major defense against mitochondrial oxidative damage, it is widely used as a marker in studies of oxidative stress, mitochondrial function and aging.
| Target | Superoxide dismutase (gene SOD2; UniProt P04179, human) |
|---|---|
| Clone | 2B12B1 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 24.8 kDa |
| Observed MW | 25 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
Samples with a confirmed band (WB): human HepG2 cells, human hcct tissue, human hccp tissue, mouse liver tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human adenocarcinoma of the right colon tissue, human liver cancer tissue, human lymphoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of human SOD2. It differs from the mouse sequence at one residue and from the rat sequence at four residues.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places superoxide dismutase in the mitochondrion matrix. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qi Zhang et al. (2016) Maternal inflammation activated ROS-p38 MAPK predisposes offspring to heart damages caused by isoproterenol via augmenting ROS generation. Scientific Reports. 10.1038/srep30146
Tian Qin et al. (2016) Sinomenine attenuates renal fibrosis through Nrf2-mediated inhibition of oxidative stress and TGFβ signaling. Toxicology and Applied Pharmacology. 10.1016/j.taap.2016.05.009
Chao Liu et al. (2024) Contribution of impaired autophagy, mitochondrial dysfunction and abnormal lipolysis to epididymal aging in mice. Experimental Gerontology. 10.1016/j.exger.2024.112528
Hyoseung Shin et al. (2016) Nonpigmented hair removal using photodynamic therapy in animal model. Lasers in Surgery and Medicine. 10.1002/lsm.22570
