Anti-Sorbitol Dehydrogenase/SORD Antibody Picoband® (monoclonal, 12B10G2)

Overview

Anti-Sorbitol Dehydrogenase/SORD Antibody Picoband® (monoclonal, 12B10G2) is an antibody for SORD detection raised in Mouse (Monoclonal, clone Clone: 12B10G2, Mouse IgG1), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: SORD (chaperonin containing TCP1 subunit 3); UniProt: Q00796
• Antibody format: Mouse, Monoclonal, clone Clone: 12B10G2, Mouse IgG1
• Molecular weight: 40 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-Sorbitol Dehydrogenase/SORD Antibody Picoband® (monoclonal, 12B10G2) catalog # M07851-1.

Biological background

Biological context: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.

Expression and localization notes: cellular localization: Cytoplasm., tissue context: Ubiquitously expressed with highest levels in spleen, thymus and immature brain..

Common research applications

• Western blotting (WB): Compare SORD levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of SORD in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify SORD-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Sorbitol dehydrogenase is an enzyme that in humans is encoded by the SORD gene. Sorbitol dehydrogenase (SORD) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase, makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications. The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cytoplasm.
• Tissue details: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
• Research category: Chaperones,Protein Trafficking,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.