| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sorbin and SH3 domain-containing protein 1; Ponsin; SH3 domain protein 5; SH3P12; c-Cbl-associated protein; CAP; SORBS1; KIAA0894; KIAA1296; SH3D5 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SORBS1 recombinant protein (Position: Q23-R323). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects sorbin and SH3 domain-containing protein 1 (gene SORBS1) in human samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 142.5 kDa, and the supplier reports an observed band at about 160 kDa.
Sorbin and SH3 domain-containing protein 1 (SORBS1, also called CAP or ponsin) is an adaptor protein that links the ubiquitin ligase CBL to the insulin receptor. This connection supports insulin-stimulated glucose uptake, and SORBS1 also takes part in forming actin stress fibers and focal adhesions, so it is studied in insulin signaling and cell adhesion.
| Target | Sorbin and SH3 domain-containing protein 1 (gene SORBS1; UniProt Q9BX66, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 142.5 kDa |
| Observed MW | 160 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human rectal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): PC-3 cells.
Immunogen
Recombinant human SORBS1 fragment (Gln23–Arg323), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places sorbin and SH3 domain-containing protein 1 in the cell junction, cell membrane, cytoplasm and nucleus. Tissue expression noted by UniProt (human): Detected in skeletal muscle (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
