| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Sorbin and SH3 domain-containing protein 1; Ponsin; SH3 domain protein 5; SH3P12; c-Cbl-associated protein; CAP; SORBS1; KIAA0894; KIAA1296; SH3D5 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | E.coli-derived human SORBS1 recombinant protein (Position: Q23-R323). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-SORBS1 Picoband® Antibody is an antibody for SORBS1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: SORBS1 (sorbin and SH3 domain containing 1); UniProt: Q9BX66; NCBI Gene: 10580
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 160 kDa, calculated 143 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-SORBS1 Picoband® Antibody catalog # A04426-3.
Biological background
Biological context: Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions.
Expression and localization notes: cellular localization: Cell membrane. Nucleus. Nucleus matrix. Cytoskeleton. Adherens junction. Focal adhesion., tissue context: Expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells..
Common research applications
- Western blotting (WB): Compare SORBS1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of SORBS1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify SORBS1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: CAP/Ponsinprotein, also known as Sorbin and SH3 domain-containing protein 1 is a protein that in humans is encoded by the SORBS1 gene. It is mapped to 10q24.1. This gene encodes a CBL-associated protein which functions in the signaling and stimulation of insulin. Mutations in this gene may be associated with human disorders of insulin resistance. Alternative splicing results in multiple transcript variants.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane. Nucleus. Nucleus matrix. Cytoskeleton. Adherens junction. Focal adhesion.
- Tissue details: Expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells.
- Research category: Cancer,Cardiovascular,Cell Type Markers,Cytokines,Growth Factors,Growth Factors/Hormones,Immunology,Innate Immunity,Signal Transduction,Tags & Cell Markers,TNF Superfamily,Vasculature
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
