| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor SOX-10; SOX10 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human SOX10, which shares 97.1% amino acid (aa) sequence identity with both mouse and rat SOX10. |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes transcription factor SOX-10 (gene SOX10) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 49.9 kDa, and the supplier reports an observed band at about 60 kDa.
Transcription factor SOX-10 (SOX10) is an HMG-box transcription factor central to neural crest derivatives and glial cells. It drives the myelination program in oligodendrocytes, including induction of MYRF and myelin genes, and together with PAX3 activates MITF in melanocytes, so SOX10 is a widely used marker for oligodendrocytes, Schwann cells and melanocytic cells.
| Target | Transcription factor SOX-10 (gene SOX10; UniProt P56693, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 49.9 kDa |
| Observed MW | 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL |
Samples with a confirmed band (WB): human SH-SY5Y cells, human U251 cells, human U2OS cells, human U87 cells, rat brain tissue, rat C6 cells, mouse brain tissue, mouse Neuro-2a whole cellIHC: human melanoma tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the middle region of human SOX10. Sequence identity with the mouse and rat orthologs: 97.1%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transcription factor SOX-10 in the cytoplasm, nucleus and mitochondrion outer membrane. Tissue expression noted by UniProt (human): Expressed in fetal brain and in adult brain, heart, small intestine and colon. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
