| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Erythroid spectrin beta ;Sptb ;LOC314251 ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Human erythrocyte spectrin. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Spectrin(Alpha And Beta) SPTB Antibody (Monoclonal, SB-SP1) is an antibody targeting SPTA1; SPTB. Common applications include WB. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: SB-SP1; isotype: Mouse IgG1; reactivity: Human; observed MW: 60 kDa; calculated MW: 246329 MW.
Boster Bio Anti-Spectrin (Alpha And Beta) SPTB Antibody (Monoclonal, SB-SP1) catalog # MA1090. Tested in WB applications. This antibody reacts with Human.
Key elements and design rationale
- Target: SPTA1; SPTB — spectrin, alpha, erythrocytic 1(elliptocytosis 2); spectrin, beta, erythrocytic
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: SB-SP1; Isotype: Mouse IgG1
- Species reactivity: Human
- Molecular weight guidance: Observed: 60 kDa; Calculated: 246329 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): SPTA1: Spectrin is the major constituent of the cytoskeletalnetwork underlying the erythrocyte plasma membrane. It associateswith band 4.1 and actin to form the cytoskeletal superstructure ofthe erythrocyte plasma membrane.|SPTB: Spectrin is the major constituent of the cytoskeletalnetwork underlying the erythrocyte plasma membrane. It associateswith band 4.1 and actin to form the cytoskeletal superstructure ofthe erythrocyte plasma membrane.
Scientific background (datasheet): Spectrin, the predominant component of the membrane skeleton of the red cell, is essential in determining the properties of the membrane including its shape and deformability. It consists of 2 nonidentical subunits, alpha and beta. Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head-to-head self-association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2-dimensional network. Non-erythroid spectrin gene is mapped to human chromosome 2. Spectrin mutations cause spinocerebellar ataxia type 5.
Cellular localization (datasheet): SPTA1: Cytoplasm, cytoskeleton. Cytoplasm, cellcortex.|Spta1: Cytoplasm, cytoskeleton. Cytoplasm, cellcortex.|SPTB: Cytoplasm, cytoskeleton. Cytoplasm, cellcortex.|Sptb: Cytoplasm, cytoskeleton. Cytoplasm, cellcortex.
Sequence similarities (datasheet): Belongs to the spectrin family.
Research relevance and current trends
- Commonly studied in contexts related to Actin, etc.,Cytoskeleton,Cytoskeleton/ECM,Microfilaments,Plasma Membrane,Signal Transduction,Subcellular Markers,Tags & Cell Markers.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
