| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CD40 ligand;CD40-L;T-cell antigen Gp39;TNF-related activation protein;TRAP;Tumor necrosis factor ligand superfamily member 5;CD154;CD40 ligand, membrane form;CD40 ligand, soluble form;CD40LG;CD40L, TNFSF5, TRAP; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human PU.1/Spi1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-SPI1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AEIG-19; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, Flow (as provided in the source record). Boster Bio Anti-SPI1 Rabbit Monoclonal Antibody catalog # M01116. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: SPI1 (CD40 ligand).
- Antibody format: Monoclonal; clone AEIG-19; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
SPI1 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. . Reported cellular localization context: Cell membrane; Single-pass type II membrane protein. Tissue expression notes (as provided): Specifically expressed on activated CD4+ T- lymphocytes.
Research relevance and current trends
- Research context keywords from the source record include: Adaptive Immunity,B Cells,DNA/RNA,Domain Families,Epigenetics and Nuclear Signaling,Immunology,Innate Immunity,Macrophage/Inflammation,Non-CD,RNA Processing,Splicing,Transcription,Transcription Factors.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate SPI1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect SPI1 expression by Western blot in cell or tissue lysates, Detect SPI1 in FFPE tissue sections by immunohistochemistry, Quantify SPI1-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 85 kDa; calculated MW: 29274 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 85 kDa
- Cellular localization (provided): Cell membrane; Single-pass type II membrane protein.
- Tissue details (provided): Specifically expressed on activated CD4+ T- lymphocytes.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
