| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine protease inhibitor Kazal-type 1; Pancreatic secretory trypsin inhibitor; Tumor-associated trypsin inhibitor; TATI; SPINK1; PSTI |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SPINK1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22S26 is a rabbit monoclonal antibody that recognizes serine protease inhibitor Kazal-type 1 (gene SPINK1) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 8.5 kDa, and the supplier reports an observed band at about 11 kDa.
Serine protease inhibitor Kazal-type 1 (SPINK1, also called PSTI or TATI) is a small secreted trypsin inhibitor. In the pancreas it guards against premature activation of trypsin and other digestive zymogens, and SPINK1 variants are a well-known risk factor for chronic pancreatitis.
| Target | Serine protease inhibitor Kazal-type 1 (gene SPINK1; UniProt P00995, human) |
|---|---|
| Clone | 22S26 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 8.5 kDa |
| Observed MW | 11 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human pancreas.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human SPINK1.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes serine protease inhibitor Kazal-type 1 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
