| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sequestosome-1;EBI3-associated protein of 60 kDa;EBIAP;p60;Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa;Ubiquitin-binding protein p62;SQSTM1;ORCA, OSIL; |
| UniProt # | |
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| Clonality | |
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| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human SQSTM1, different from the related rat and mouse sequences by one amino acid. |
| Molecular weight | |
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| Reconstitution | |
| Cellular localization | |
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| Form | Lyophilized |
| Storage | |
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Product Overview
This rabbit polyclonal antibody detects sequestosome-1 (gene SQSTM1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 47.7 kDa, and the supplier reports an observed band at about 60 kDa.
Sequestosome-1 (SQSTM1, also known as p62) is an autophagy receptor that binds polyubiquitinated proteins and LC3 on autophagosomes, delivering protein aggregates for degradation. It also acts as a signaling hub for pathways such as KEAP1-NRF2, and because it is itself degraded by autophagy, p62 is widely used as a marker of autophagic flux.
| Target | Sequestosome-1 (gene SQSTM1; UniProt Q13501, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 47.7 kDa |
| Observed MW | 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human COLO-320 cells, human A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): rat brain tissue, human gastric cancer tissue, human lung cancer tissue, human oesophagus squama cancer tissue, human rectal cancer tissue, human sarcoma tissue, human cholangiocarcinoma tissue, human liver cancer tissue, mouse brain tissue, rat liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with a confirmed signal (flow cytometry): A549 cells.
Immunogen
Synthetic peptide from the N-terminal region of human SQSTM1. It differs from the rat and mouse sequences at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sequestosome-1 in the cytoplasmic vesicle, preautophagosomal structure, cytoplasm and nucleus. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
