| Field | Specification |
|---|---|
| Target | |
| Alternative names | Proto-oncogene tyrosine-protein kinase Src;2.7.10.2;Proto-oncogene c-Src;pp60c-src;p60-Src;SRC;SRC1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Src |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone III-19 is a rabbit monoclonal antibody that recognizes proto-oncogene tyrosine-protein kinase Src in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 59.8 kDa, and the supplier reports an observed band at about 60 kDa.
Src is a non-receptor tyrosine kinase that relays signals from integrins, growth factor receptors and other cell-surface receptors to pathways controlling adhesion, migration, proliferation and survival. Autophosphorylation at Tyr419 in the activation loop (Tyr416 in chicken numbering) marks the active kinase, so phospho-Tyr419 antibodies are used as a readout of Src activity.
| Target | Proto-oncogene tyrosine-protein kinase Src (gene SRC; UniProt P12931, human) |
|---|---|
| Clone | III-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 59.8 kDa |
| Observed MW | 60 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human 293T cells, human HeLa cells, human K562 cells, rat brain tissue, rat spleen tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue, human liver cancer tissue, mouse kidney tissue, rat testis tissue, human stomach cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): human prostate cancer tissue, human stomach cancer tissue.
Immunogen
Synthetic peptide based on the sequence of human src.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places proto-oncogene tyrosine-protein kinase Src in the cell membrane, mitochondrion inner membrane, nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed ubiquitously. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Min Hao et al. (2022) Active ingredients screening and pharmacological mechanism research of curcumae rhizoma-sparganii rhizoma herb pair ameliorates liver fibrosis based on network pharmacology. Journal of Ethnopharmacology. 10.1016/j.jep.2022.116111
