| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | SRC kinase signaling inhibitor 1; SNAP-25-interacting protein; SNIP; p130Cas-associated protein; p140Cap; SRCIN1; KIAA1684; P140 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human SRCIN1 recombinant protein (Position: E189-E287). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-SRCIN1 Antibody Picoband® is an antibody for SRCIN1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IHC-F, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: SRCIN1 (SRC kinase signaling inhibitor 1); UniProt: Q9C0H9
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 140 kDa, calculated 17136 MW
- Applications: WB, IHC, IHC-F, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-SRCIN1 Antibody Picoband® catalog # A08110-1.
Biological background
Biological context: Acts as a negative regulator of SRC by activating CSK which inhibits SRC activity and downstream signaling, leading to impaired cell spreading and migration. Regulates dendritic spine morphology. Involved in calcium-dependent exocytosis. May play a role in neurotransmitter release or synapse maintenance.
Expression and localization notes: cellular localization: Postsynaptic density. Cytoskeleton. Cytoplasm. Axon. Dendrite. Synapse., tissue context: Expressed in some primary breast carcinomas where its presence is significantly associated with increased tumor size. Not detected in normal breast tissue..
Common research applications
- Western blotting (WB): Compare SRCIN1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of SRCIN1 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify SRCIN1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Using yeast 2-hybrid analysis, protein pull-down assays, and mutation analysis, it is showed that the first coiled-coil domain of rat Snip interacted with the N-terminal t-SNARE domain of Snap25 (600322). Biochemical studies demonstrated that Snip was tightly associated with rat brain cytoskeleton. In immunofluorescence and confocal microscopy of rat PC12 pheochromocytoma cells revealed colocalization of Snip with Snap25 in the actin cytoskeleton, particularly in filopodia, lamellipodia, and neuritic extensions, including the tips. Overexpression of Snip or its Snap25-interacting domain inhibited calcium-dependent exocytosis from PC12 cells. It is concluded that SNIP is involved in regulation of neurosecretion, perhaps via its interaction with SNAP25 and the cytoskeleton.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Postsynaptic density. Cytoskeleton. Cytoplasm. Axon. Dendrite. Synapse.
- Tissue details: Expressed in some primary breast carcinomas where its presence is significantly associated with increased tumor size. Not detected in normal breast tissue.
- Research category: Co-Factors,Epigenetics and Nuclear Signaling,Mediator Complex,Pol II Transcription,Polymerase Associated Factors,Transcription
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
