| Field | Specification |
|---|---|
| Target | |
| Alternative names | Importin subunit alpha-5; Karyopherin subunit alpha-1; Nucleoprotein interactor 1; NPI-1; RAG cohort protein 2; SRP1-beta; KPNA1; RCH2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human SRP1/KPNA1, which shares 89.3% and 92.9% amino acid (aa) sequence identity with mouse and rat SRP1/KPNA1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects importin subunit alpha-5 (gene KPNA1) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 60.2 kDa, and the supplier reports an observed band at about 60 kDa.
Importin α5 (KPNA1) is an adapter for nuclear protein import. It recognizes classical nuclear localization signals on cargo proteins and binds importin β1, which carries the complex through the nuclear pore; RanGTP in the nucleus then releases the cargo. KPNA1 is known for importing STAT1 after interferon signaling and is studied in nuclear transport and virus–host interactions.
| Target | Importin subunit alpha-5 (gene KPNA1; UniProt P52294, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 60.2 kDa |
| Observed MW | 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HL-60 cells, human Caco-2 cells, human PC-3 cells, rat heart tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Synthetic peptide from the N-terminal region of human SRP1/KPNA1. Sequence identity with the mouse and rat orthologs: 89.3% and 92.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places importin subunit alpha-5 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed ubiquitously. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ming Ying et al. (2007) Nuclear import of human sexual regulator DMRT1 is mediated by importin-β. Biochimica et Biophysica ACTA-Molecular Cell Research. 10.1016/j.bbamcr.2007.03.006
