| Field | Specification |
|---|---|
| Target | |
| Alternative names | Signal recognition particle subunit SRP54; Signal recognition particle 54 kDa protein; SRP54 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human SRP54 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20S91 is a rabbit monoclonal antibody that recognizes signal recognition particle subunit SRP54 in human, mouse and rat samples and is validated for Western blot, IHC, immunoprecipitation and flow cytometry. The predicted molecular weight is 55.7 kDa, and the supplier reports an observed band at about 54 kDa.
Signal recognition particle subunit SRP54 is the GTPase of the signal recognition particle. It recognizes signal sequences as they emerge from the ribosome and, through GTP-dependent interaction with the SRP receptor, delivers nascent secretory and membrane proteins to the ER membrane; SRP54 mutations are linked to congenital neutropenia.
| Target | Signal recognition particle subunit SRP54 (gene SRP54; UniProt P61011, human) |
|---|---|
| Clone | 20S91 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 55.7 kDa |
| Observed MW | 54 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human SRP54.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places signal recognition particle subunit SRP54 in the nucleus speckle, cytoplasm and endoplasmic reticulum. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
