| Field | Specification |
|---|---|
| Target | |
| Alternative names | Stathmin;Leukemia-associated phosphoprotein p18;Metablastin;Oncoprotein 18;Op18;Phosphoprotein p19;pp19;Prosolin;Protein Pr22;pp17;STMN1;C1orf215, LAP18, OP18; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Stathmin 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DDA-19 is a rabbit monoclonal antibody that recognizes stathmin (gene STMN1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 17.3 kDa, and the supplier reports an observed band at about 17 kDa.
Stathmin (STMN1, also called oncoprotein 18) is a small cytosolic phosphoprotein that destabilizes microtubules by sequestering tubulin dimers and promoting microtubule catastrophe. Its activity is switched off by phosphorylation, including at Ser16, linking it to cell cycle progression, neuronal development and cell migration, and it is often highly expressed in cancers.
| Target | Stathmin (gene STMN1; UniProt P16949, human) |
|---|---|
| Clone | DDA-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 17.3 kDa |
| Observed MW | 17 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human MDA-MB-453 cells, human SH-SY5Y cells, human Raji cells, rat brain tissue, rat testis tissue, rat PC-12 cells, mouse brain tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human colon tissue, human colon cancer tissue, human ovarian cancer tissue, neuroplexus of human colon tissue, neuroplexus of rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human stathmin 1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places stathmin in the cytoplasm. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhi-Hui Luo et al. (2026) Spatial transcriptomic-metabolic features of tumor foci and tumor capsule in microvascular invasion with hepatocellular carcinoma: A spatial multi-omics study. PLOS Medicine. 10.1371/journal.pmed.1004703
