| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Steryl-sulfatase; Arylsulfatase C; ASC; Steroid sulfatase; Steryl-sulfate sulfohydrolase; STS; ARSC1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human Steroid sulfatase recombinant protein (Position: K297-E396). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of STS (Cytoglobin) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Steroid sulfatase/STS Antibody Picoband® catalog # A01198-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human Steroid sulfatase recombinant protein (Position: K297-E396). (reported region: K297-E396).
- Molecular weight context: reported MW: 65 kDa; calculated MW: 21405 MW
- Reactivity: Human,Mouse,Rat
- Applications: ELISA, Flow Cytometry, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Cytoglobin; steroid sulfatase. Steroid sulfatase (STS), or steryl-sulfatase, formerly known as arylsulfatase C, is a sulfatase enzyme involved in the metabolism of steroids. It is encoded by the STS gene. This gene encodes a multi-pass membrane protein that is localized to the endoplasmic reticulum. It belongs to the sulfatase family and hydrolyzes several 3-beta-hydroxysteroid sulfates, which serve as metabolic precursors for estrogens, androgens, and cholesterol. Mutations in this gene are associated with X-linked ichthyosis (XLI). Alternatively spliced transcript variants resulting from the use of different promoters have been described for this gene. Functional note: Conversion of sulfated steroid precursors to estrogens during pregnancy. Reported localization: Endoplasmic reticulum membrane; Multi-pass membrane protein. Expression/tissue context: Ubiquitously expressed. Highest expression in heart, stomach, bladder and small intestine. .
Research relevance and current trends
- Adapters: Researchers commonly examine how STS (Cytoglobin) relates to this theme using model systems and orthogonal readouts.
- Adaptive Immunity: Researchers commonly examine how STS (Cytoglobin) relates to this theme using model systems and orthogonal readouts.
- Immunology: Researchers commonly examine how STS (Cytoglobin) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative STS (Cytoglobin) levels across conditions; band patterns may reflect isoforms and processing.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
