| Field | Specification |
|---|---|
| Target | |
| Alternative names | Stress-induced-phosphoprotein 1;STI1;Hsc70/Hsp90-organizing protein;Hop;Renal carcinoma antigen NY-REN-11;Transformation-sensitive protein IEF SSP 3521;STIP1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human STIP1, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes stress-induced-phosphoprotein 1 (gene STIP1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 62.6 kDa, and the supplier reports an observed band at about 63 kDa.
Stress-induced phosphoprotein 1 (STIP1, also called HOP) is a TPR-domain co-chaperone that links HSP70/HSC70 and HSP90. By bridging the two chaperones it helps transfer client proteins such as steroid receptors and kinases from HSP70 to HSP90, and it is studied in protein folding and proteostasis.
| Target | Stress-induced-phosphoprotein 1 (gene STIP1; UniProt P31948, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 62.6 kDa |
| Observed MW | 63 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human HeLa cells, human COLO320 cells, human MCF-7 cells, rat C6 cells, rat RH35 cells, mouse brain tissue, mouse liver tissue, mouse Neuro-2a cells, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human STIP1. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places stress-induced-phosphoprotein 1 in the cytoplasm, nucleus and dynein axonemal particle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jiang Wang et al. (2017) Autocrine and paracrine STIP1 signaling promote osteolytic bone metastasis in renal cell carcinoma. Oncotarget. 10.18632/oncotarget.15222
