Anti-STK3/MST-2 Antibody Picoband®

Overview

Anti-STK3/MST-2 Antibody Picoband® is an antibody for STK3 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Monkey. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: STK3 (colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)); UniProt: Q13188
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 56 kDa, calculated 38160 MW
• Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-STK3/MST-2 Antibody Picoband® catalog # A02224-2.

Biological background

Biological context: High affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.

Expression and localization notes: cellular localization: Membrane. Single-pass type I membrane protein., tissue context: Broadly expressed with higher expression in heart..

Common research applications

• Western blotting (WB): Compare STK3 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of STK3 in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify STK3-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Serine/threonine-protein kinase 3 is an enzyme that in humans is encoded by the STK3 gene. This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Membrane. Single-pass type I membrane protein.
• Tissue details: Broadly expressed with higher expression in heart.
• Research category: Auditory System,Domain Families,Epigenetics and Nuclear Signaling,Hlh/Leucine Zipper,Neurogenesis,Neurology Process,Neuroscience,Nuclear,Sensory System,Signaling Pathways,Stem Cells,Transcription

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.