| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase 33; STK33 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human STK33 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22S21 is a rabbit monoclonal antibody that recognizes serine/threonine-protein kinase 33 (gene STK33) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 57.8 kDa, and the supplier reports an observed band at about 58 kDa.
STK33 is a serine/threonine kinase of the calcium/calmodulin-dependent kinase group that is highly expressed in the testis. It is needed for spermatid differentiation and sperm flagellum assembly, acting by phosphorylating the sperm fibrous sheath components AKAP3 and AKAP4, and it also phosphorylates vimentin to influence intermediate filament dynamics.
| Target | Serine/threonine-protein kinase 33 (gene STK33; UniProt Q9BYT3, human) |
|---|---|
| Clone | 22S21 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 57.8 kDa |
| Observed MW | 58 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human 293T cells, human U251 cells, human Jurkat cells, human U2OS cells, human HeLa cells, human HepG2 cells, human A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human STK33.
Reactivity Notes
The supplier lists reactivity with human. UniProt places serine/threonine-protein kinase 33 in the cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in testis, fetal lung and heart, followed by pituitary gland, kidney, interventricular septum, pancreas, heart, trachea, thyroid gland and uterus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
