| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein Stra8 ;Stra8 ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of rat Stra8, different from the related mouse sequence by one amino acid. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Stra8 Antibody Picoband® is an antibody targeting STRA8. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Rat; observed MW: 37 kDa; calculated MW: 41262 MW.
Boster Bio Anti-Stra8 Antibody catalog # PA1528. Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: STRA8 — stimulated by retinoic acid gene 8 homolog(mouse)
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
- Species reactivity: Rat
- Molecular weight guidance: Observed: 37 kDa; Calculated: 41262 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. .
Scientific background (datasheet): Stra8, stimulated by retinoic acid 8, is expressed in embryonic ovaries just before meiotic initiation, whereas in testis it is expressed after birth. The STRA8 gene contains 9 exons. And this gene is mapped to chromosome 7q31. Retinoic acid (RA) signaling was required for Stra8 expression, and therefore initiation of meiosis, in embryonic mouse ovaries. RA was sufficient to induce Stra8 expression in embryonic testis. Cyp26-mediated RA metabolism was responsible for delaying Stra8 expression in embryonic testis. Since RA signaling regulated Stra8 expression in both embryonic ovaries and adult testis, it was said this portion of the meiotic initiation pathway may be identical in both sexes.
Cellular localization (datasheet): Cell membrane ; Single-pass type I membrane protein . Melanosome . Colocalizes with SLC16A1 and SLC16A8. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .
Tissue details (datasheet): Present only in vascular endothelium in non- neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
Sequence similarities (datasheet): Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Research relevance and current trends
- Commonly studied in contexts related to Germ Cell Markers,Germline Stem Cells,Intracellular,Reproduction,Stem Cells,Surface Molecules.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
