| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Small ubiquitin-related modifier 1;SUMO-1;GAP-modifying protein 1;GMP1;SMT3 homolog 3;Sentrin;Ubiquitin-homology domain protein PIC1;Ubiquitin-like protein SMT3C;Smt3C;Ubiquitin-like protein UBL1;SUMO1;SMT3C, SMT3H3, UBL1;OK/SW-cl.43; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Sumo 1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-SUMO1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone BFI-19; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-Sumo 1 Rabbit Monoclonal Antibody catalog # M00631-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: SUMO1 (Small ubiquitin-related modifier 1).
- Antibody format: Monoclonal; clone BFI-19; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
SUMO1 (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1 (PubMed:19223394). Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. . Reported cellular localization context: Nucleus membrane. Nucleus speckle. Cytoplasm. Nucleus, PML body. Cell membrane . Recruited by BCL11A into the nuclear body. . Tissue expression notes (as provided): Fetal muscle and adult liver, brain and thyroid.
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Cardiovascular,Cell Biology,Cell Death,Metabolism,Metabolism Processes,Pathways and Processes,Proteasome / Ubiquitin,Proteolysis/Ubiquitin.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate SUMO1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect SUMO1 expression by Western blot in cell or tissue lysates, Detect SUMO1 in FFPE tissue sections by immunohistochemistry, Localize SUMO1 by immunofluorescence/immunocytochemistry in cultured cells, Quantify SUMO1-positive cells by flow cytometry in single-cell suspensions, Enrich SUMO1 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 26 kDa; calculated MW: 11557 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 26 kDa
- Cellular localization (provided): Nucleus membrane. Nucleus speckle. Cytoplasm. Nucleus, PML body. Cell membrane . Recruited by BCL11A into the nuclear body. .
- Tissue details (provided): Fetal muscle and adult liver, brain and thyroid.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
