| Field | Specification |
|---|---|
| Target | |
| Alternative names | Small ubiquitin-related modifier 1;SUMO-1;GAP-modifying protein 1;GMP1;SMT3 homolog 3;Sentrin;Ubiquitin-homology domain protein PIC1;Ubiquitin-like protein SMT3C;Smt3C;Ubiquitin-like protein UBL1;SUMO1;SMT3C, SMT3H3, UBL1;OK/SW-cl.43; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Sumo 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BFI-19 is a rabbit monoclonal antibody that recognizes small ubiquitin-related modifier 1 (gene SUMO1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 11.6 kDa; the supplier reports observed bands at 18, 80 kDa.
SUMO-1 is a small ubiquitin-like modifier that is attached to lysine residues of target proteins through the E1 SAE1/SAE2, the E2 UBC9 (UBE2I) and E3 ligases such as PIAS proteins. SUMOylation changes protein localization, interactions and stability, regulating nuclear transport, transcription, DNA repair and mitosis; the classic SUMO-1 target is RanGAP1 at the nuclear pore.
| Target | Small ubiquitin-related modifier 1 (gene SUMO1; UniProt P63165, human) |
|---|---|
| Clone | BFI-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 11.6 kDa |
| Observed MW | 18, 80 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human MCF-7 cells, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human sumo 1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places small ubiquitin-related modifier 1 in the nuclear membrane, nucleus speckle, cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
