| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Synaptophysin;Major synaptic vesicle protein p38;Syp; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Rat retina synaptosome. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Synaptophysin Syp Antibody (Monoclonal, SVP-38) is an antibody targeting SYP. Common applications include WB, IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: SVP-38; isotype: Mouse IgG1; reactivity: Human,Rat; observed MW: 60 kDa; calculated MW: 33311 MW.
Boster Bio Anti-Synaptophysin Syp Antibody (Monoclonal, SVP-38) catalog # MA1091. Tested in IHC, WB applications. This antibody reacts with Human, Rat.
Key elements and design rationale
- Target: SYP — Synaptophysin
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: SVP-38; Isotype: Mouse IgG1
- Species reactivity: Human,Rat
- Molecular weight guidance: Observed: 60 kDa; Calculated: 33311 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity). .
Scientific background (datasheet): Synaptophysin (SYP) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells. Ozcelik et al. (1990) concluded that the gene has 7 exons distributed over about 20 kb. SYP mapped the SYP locus to Xp11.23-p11.22. Regionalization of the gene on the X chromosome was also done with hamster/human hybrid cells in which various portions of the human X chromosome were present.
Cellular localization (datasheet): Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome.
Tissue details (datasheet): Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
Sequence similarities (datasheet): Belongs to the synaptophysin/synaptobrevin family.
Research relevance and current trends
- Commonly studied in contexts related to Actin, etc.,Cytoskeleton,Cytoskeleton/ECM,Microfilaments,Plasma Membrane,Signal Transduction,Subcellular Markers,Tags & Cell Markers.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
