| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cell adhesion molecule 1;Immunoglobulin superfamily member 4;IgSF4;Nectin-like protein 2;NECL-2;Spermatogenic immunoglobulin superfamily;SgIgSF;Synaptic cell adhesion molecule;SynCAM;Tumor suppressor in lung cancer 1;TSLC-1;CADM1 ;IGSF4 , IGSF4A, NECL2 , SYNCAM , TSLC1 ; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SynCAM recombinant protein (Position: Q45-P340). Human SynCAM shares 99% amino acid (aa) sequence identity with mouse SynCAM. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes cell adhesion molecule 1 (gene CADM1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 48.5 kDa, and the supplier reports an observed band at about 49 kDa.
Cell adhesion molecule 1 (CADM1, also called SynCAM1 or TSLC1) is an immunoglobulin-superfamily adhesion protein. It mediates homophilic and heterophilic cell–cell contacts, helps organize synapses in the brain, and its binding to CRTAM promotes NK and CD8 T-cell activity. It has also been described as a tumor suppressor in lung and other cancers.
| Target | Cell adhesion molecule 1 (gene CADM1; UniProt Q9BY67, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 48.5 kDa |
| Observed MW | 49 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human hepatocellular carcinoma tumor tissue (HCCT), human hepatocellular carcinoma paracancerous tissue (HCCP), human HepG2 cells, rat liver tissue, mouse brain tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human SynCAM fragment (Gln45–Pro340), expressed in E. coli. Sequence identity with the mouse ortholog: 99%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cell adhesion molecule 1 in the cell membrane and synapse. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
