| Field | Specification |
|---|---|
| Target | |
| Alternative names | TRAF family member-associated NF-kappa-B activator; TRAF-interacting protein; I-TRAF; TANK; ITRAF; TRAF2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human TANK, which shares 93.9% amino acid (aa) sequence identity with both mouse and rat TANK. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects TRAF family member-associated NF-kappa-B activator (gene TANK) in human, mouse and rat samples and is validated for flow cytometry, IHC and Western blot. The predicted molecular weight is 47.8 kDa, and the supplier reports an observed band at about 48 kDa.
TANK (also called I-TRAF) is an adaptor that binds TBK1 and IKKε and associates with TRAF proteins. It contributes to antiviral interferon responses while also restraining NF-κB activation, in part by helping deubiquitinating enzymes remove activating ubiquitin chains from TRAF6 and NEMO.
| Target | TRAF family member-associated NF-kappa-B activator (gene TANK; UniProt Q92844, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 47.8 kDa |
| Observed MW | 48 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| IHC (frozen sections) | 0.5–1 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): rat brain tissue, rat lung tissue, rat spleen tissue, rat kidney tissue, mouse brain tissue, mouse lung tissue, mouse spleen tissue, mouse kidney tissue, human HeLa cells, human A549 cells, human MDA-MB-453 cells, human SW620 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat small intestine tissue, human cholangiocarcinoma tissue, human placenta tissue, rat spleen tissue, human rectal cancer tissue, mouse kidney tissue, mouse liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (IHC): human placenta tissue.
Samples with a confirmed signal (flow cytometry): A431 cells.
Immunogen
Synthetic peptide from the N-terminal region of human TANK. Sequence identity with the mouse and rat orthologs: 93.9%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places TRAF family member-associated NF-kappa-B activator in the cytoplasm. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
