| Field | Specification |
|---|---|
| Target | |
| Alternative names | Antigen peptide transporter 1;APT1;ATP-binding cassette sub-family B member 2;Peptide supply factor 1;Peptide transporter PSF1;PSF-1;Peptide transporter TAP1;Peptide transporter involved in antigen processing 1;Really interesting new gene 4 protein;TAP1;ABCB2, PSF1, RING4, Y3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human TAP1, different from the related mouse sequence by eight amino acids, and from the related rat sequence by nine amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets antigen peptide transporter 1 (gene TAP1) in human samples and is validated for IHC and Western blot. The predicted molecular weight is 87.2 kDa, and the supplier reports an observed band at about 87 kDa.
TAP1 is one half of the transporter associated with antigen processing, an ATP-binding cassette transporter in the ER membrane formed with TAP2. It pumps short cytosolic peptides into the ER for loading onto MHC class I molecules, making it central to antigen presentation to CD8+ T cells and a common target of viral immune evasion.
| Target | Antigen peptide transporter 1 (gene TAP1; UniProt Q03518, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 87.2 kDa |
| Observed MW | 87 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): HeLa cells, HUT cells, SW620 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 40 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human intestinal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the middle region of human TAP1. It differs from the mouse sequence at eight residues and from the rat sequence at nine residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt places antigen peptide transporter 1 in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Highly expressed in professional APCs monocytes and dendritic cells as well as in lymphocyte subsets T cells, B cells and NK cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
