| Field | Specification |
|---|---|
| Target | |
| Alternative names | Microtubule-associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; MAPT; MAPTL; MTBT1; TAU |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Tau/MAPT recombinant protein (Position: M1-L322). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects microtubule-associated protein tau (gene MAPT) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and ELISA. The predicted molecular weight is 78.9 kDa, and the supplier reports an observed band at about 50–70 kDa.
Tau (MAPT) is a microtubule-associated protein enriched in neuronal axons, where it binds and stabilizes microtubules and helps maintain axonal transport. Abnormal phosphorylation causes tau to detach from microtubules and form aggregates, the hallmark of neurofibrillary tangles in Alzheimer′s disease and other tauopathies, which makes it one of the most studied proteins in neurodegeneration research.
| Target | Microtubule-associated protein tau (gene MAPT; UniProt P10636, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 78.9 kDa |
| Observed MW | 50–70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human brain tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): T-47D cells.
Immunogen
Recombinant human Tau/MAPT fragment (Met1–Leu322), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places microtubule-associated protein tau in the cytoplasm, cell membrane and cell projection. Tissue expression noted by UniProt (human): Expressed in neurons. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qin Tu et al. (2011) The effect of acetylcholine-like biomimetic polymers on neuronal growth. Biomaterials. 10.1016/j.biomaterials.2011.01.044
Li Li et al. (2012) Spatiotemporally Controlled and Multifactor Involved Assay of Neuronal Compartment Regeneration after Chemical Injury in an Integrated Microfluidics. Analytical Chemistry. 10.1021/ac3013708
Yushi Zhao et al. (2026) IL-1β neutralization ameliorates cognitive deficits and tau pathology in a mouse model of Alzheimer's disease with hyperhomocysteinemia. Experimental Neurology. 10.1016/j.expneurol.2026.115729
Li Ruomeng et al. (2026) Bushen Huoxue Acupuncture alleviates alzheimer’s disease progression via the E3 ubiquitin ligase SMURF2‑mediated ubiquitination of LAPTM5. Metabolic Brain Disease. 10.1007/s11011-026-01902-z
Ji Kai-Hong et al. (2008) Simultaneous expression of Oct4 and genes of three germ layers in single cell-derived multipotent adult progenitor cells. Annals of Hematology. 10.1007/s00277-008-0470-3
Xi Li et al. (2012) Cellular and molecular mechanisms underlying the action of ginsenoside Rg1 against Alzheimer's disease. Neural Regeneration Research. 10.3969/j.issn.1673-5374.2012.36.002
