| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tax1-binding protein 3; Glutaminase-interacting protein 3; Tax interaction protein 1; TIP-1; Tax-interacting protein 1; TAX1BP3; TIP1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TAX1BP3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADDC-20 is a rabbit monoclonal antibody that recognizes tax1-binding protein 3 (gene TAX1BP3) in human, mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 13.7 kDa, and the supplier reports an observed band at about 14 kDa.
TAX1BP3 (also called TIP-1) is a small protein consisting almost entirely of a single PDZ domain. By competing for PDZ-binding sites it can regulate several protein interactions, including binding to β-catenin to restrain Wnt signaling, and it was first identified as a partner of the HTLV-1 Tax protein.
| Target | Tax1-binding protein 3 (gene TAX1BP3; UniProt O14907, human) |
|---|---|
| Clone | ADDC-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 13.7 kDa |
| Observed MW | 14 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HT1080 cells, human SIHA cells, human A549 cells, human Hacat cells, rat small intestine tissue, rat lung tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human TAX1BP3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tax1-binding protein 3 in the cytoplasm, nucleus and cell membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
