| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | TATA-box-binding protein;TATA sequence-binding protein;TATA-binding factor;TATA-box factor;Transcription initiation factor TFIID TBP subunit;TBP;GTF2D1, TF2D, TFIID; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human TBP |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal Antibody is an antibody targeting TBP. Common applications include WB. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: DOA-20; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 62 kDa; calculated MW: 37698 MW.
Boster Bio Anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal Antibody catalog # M00302. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: TBP — TATA-box-binding protein
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: DOA-20; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 62 kDa; Calculated: 37698 MW
Biological background
Protein function (datasheet): General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre- initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. .
Cellular localization (datasheet): Nucleus.
Tissue details (datasheet): Widely expressed, with levels highest in the testis and ovary. .
Research relevance and current trends
- Commonly studied in contexts related to 2339,Epigenetics and Nuclear Signaling,Pol II Transcription,Polymerase Associated Factors,Transcription.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
