| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor 4; TCF-4; Class B basic helix-loop-helix protein 19; bHLHb19; Immunoglobulin transcription factor 2; ITF-2; SL3-3 enhancer factor 2; SEF-2; TCF4; ITF2; SEF2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TCF4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25T08 is a rabbit monoclonal antibody that recognizes transcription factor 4 (gene TCF4) in human samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 71.3 kDa; the supplier reports observed bands at 71, 90 kDa.
TCF4 (also called E2-2) is a class I basic helix-loop-helix E-protein that binds E-box motifs, alone or with other bHLH partners. It is important for neuronal differentiation and for plasmacytoid dendritic cell development, and TCF4 haploinsufficiency causes Pitt-Hopkins syndrome; it should not be confused with TCF7L2, which is also sometimes called TCF4.
| Target | Transcription factor 4 (gene TCF4; UniProt P15884, human) |
|---|---|
| Clone | 25T08 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 71.3 kDa |
| Observed MW | 71, 90 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:80 |
Samples with a confirmed band (WB): SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human TCF4.
Reactivity Notes
The supplier lists reactivity with human. UniProt places transcription factor 4 in the nucleus. Tissue expression noted by UniProt (human): Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
