| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Transcription factor 7-like 1;HMG box transcription factor 3;TCF-3;TCF7L1;TCF3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human TCF7L1, different from the related mouse sequence by one amino acid. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of TCF7L1 (Transcription factor 7-like 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-TCF7L1 Antibody Picoband® catalog # PB9897. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human TCF7L1, different from the related mouse sequence by one amino acid.
- Molecular weight context: reported MW: 63 kDa; calculated MW: 62631 MW
- Reactivity: Human
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Transcription factor 7-like 1; Transcription factor 7-like 1. Transcription factor 7-like 1 (T-cell specific, HMG-box), also known as TCF7L1, is a human gene. This gene encodes a member of the T cell factor/lymphoid enhancer factor family of transcription factors. These transcription factors are activated by beta catenin, mediate the Wnt signaling pathway and are antagonized by the transforming growth factor beta signaling pathway. The encoded protein contains a high mobility group-box DNA binding domain and participates in the regulation of cell cycle genes and cellular senescence. Functional note: Participates in the Wnt signaling pathway. Binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. Necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis (By similarity). Down-regulates NQO1, leading to increased mitomycin c resistance. . Reported localization: Nucleus. Expression/tissue context: Detected in hair follicles and skin keratinocytes, and at lower levels in stomach epithelium. .
Research relevance and current trends
- Chromatin Binding Proteins: Researchers commonly examine how TCF7L1 (Transcription factor 7-like 1) relates to this theme using model systems and orthogonal readouts.
- DNA/RNA Binding: Researchers commonly examine how TCF7L1 (Transcription factor 7-like 1) relates to this theme using model systems and orthogonal readouts.
- Domain Families: Researchers commonly examine how TCF7L1 (Transcription factor 7-like 1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative TCF7L1 (Transcription factor 7-like 1) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of TCF7L1 (Transcription factor 7-like 1) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
