Anti-TCP1 alpha Antibody Picoband® (monoclonal, 2E7)

Overview

This antibody is intended for detection of TCP1 (T-complex protein 1 subunit alpha) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-TCP1 alpha Antibody Picoband® (monoclonal, 2E7) catalog # M02389. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Mouse Monoclonal Mouse IgG1
• Clone number: Clone: 2E7
• Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human TCP1 alpha, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
• Molecular weight context: reported MW: 60 kDa; calculated MW: nan
• Reactivity: Human
• Applications: Flow Cytometry, IF, IHC, ICC, WB

As a monoclonal antibody, the reagent targets a defined epitope, supporting consistency across experiments; epitope masking by PTMs or conformational changes can affect signal.

Biological background

T-complex protein 1 subunit alpha; t-complex 1. T-complex protein 1 subunit alpha is a protein that in humans is encoded by the TCP1 gene. The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found. Functional note: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Reported localization: Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Expression/tissue context: Widely expressed. Highest level in placenta, liver, muscle and kidney.

Research relevance and current trends

• Actin: Researchers commonly examine how TCP1 (T-complex protein 1 subunit alpha) relates to this theme using model systems and orthogonal readouts.
• Actin: Researchers commonly examine how TCP1 (T-complex protein 1 subunit alpha) relates to this theme using model systems and orthogonal readouts.
• etc.: Researchers commonly examine how TCP1 (T-complex protein 1 subunit alpha) relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative TCP1 (T-complex protein 1 subunit alpha) levels across conditions; band patterns may reflect isoforms and processing.
• IHC/IHC-F: assess spatial distribution of TCP1 (T-complex protein 1 subunit alpha) across tissue regions and cell types using matched controls.
• IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
• Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.