| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | T-complex protein 1 subunit delta;TCP-1-delta;CCT-delta;Stimulator of TAR RNA-binding;CCT4;CCTD, SRB; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human CCT4 recombinant protein (Position: R452-R539). Human CCT4 shares 97.7% amino acid (aa) sequence identity with both mouse and rat CCT4. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CCT4 (T-complex protein 1 subunit delta) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-TCP1 delta/CCT4 Antibody Picoband® catalog # PB9927. Tested in Flow Cytometry, IP, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human CCT4 recombinant protein (Position: R452-R539). Human CCT4 shares 97.7% amino acid (aa) sequence identity with both mouse and rat CCT4. (reported region: R452-R539).
- Molecular weight context: reported MW: 58 kDa; calculated MW: 57924 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IP, IF, IHC, IHC-F, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
T-complex protein 1 subunit delta; T-complex protein 1 subunit delta. T-complex protein 1 subunit delta is a protein that in humans is encoded by the CCT4 gene. This gene is mapped to 2p15. The chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha-transducin. Functional note: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. . Reported localization: Cytoplasm . Melanosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, cilium basal body . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. . Expression/tissue context: Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta. .
Research relevance and current trends
- Chaperones: Researchers commonly examine how CCT4 (T-complex protein 1 subunit delta) relates to this theme using model systems and orthogonal readouts.
- Protein Trafficking: Researchers commonly examine how CCT4 (T-complex protein 1 subunit delta) relates to this theme using model systems and orthogonal readouts.
- Signal Transduction: Researchers commonly examine how CCT4 (T-complex protein 1 subunit delta) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CCT4 (T-complex protein 1 subunit delta) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of CCT4 (T-complex protein 1 subunit delta) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
