| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | T-complex protein 1 subunit epsilon;TCP-1-epsilon;CCT-epsilon;CCT5;CCTE, KIAA0098; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human CCT5 recombinant protein (Position: L443-K535). Human CCT5 shares 95.7% amino acid (aa) sequence identity with both mouse and rat CCT5. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CCT5 (T-complex protein 1 subunit epsilon) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-TCP1 epsilon/CCT5 Antibody Picoband® catalog # PB9928. Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human CCT5 recombinant protein (Position: L443-K535). Human CCT5 shares 95.7% amino acid (aa) sequence identity with both mouse and rat CCT5. (reported region: L443-K535).
- Molecular weight context: reported MW: 60 kDa; calculated MW: 59671 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IP, IF, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
T-complex protein 1 subunit epsilon; T-complex protein 1 subunit epsilon. CCT5 is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Mutations in this gene cause hereditary sensory and autonomic neuropathy with spastic paraplegia (HSNSP). Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 13. Functional note: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. . Reported localization: Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Expression/tissue context: Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta. .
Research relevance and current trends
- Actin Assembly: Researchers commonly examine how CCT5 (T-complex protein 1 subunit epsilon) relates to this theme using model systems and orthogonal readouts.
- Actin: Researchers commonly examine how CCT5 (T-complex protein 1 subunit epsilon) relates to this theme using model systems and orthogonal readouts.
- etc.: Researchers commonly examine how CCT5 (T-complex protein 1 subunit epsilon) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CCT5 (T-complex protein 1 subunit epsilon) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of CCT5 (T-complex protein 1 subunit epsilon) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
