| Field | Specification |
|---|---|
| Target | |
| Alternative names | TAR DNA-binding protein 43;TDP-43;TARDBP;TDP43; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TDP43 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26T75 is a rabbit monoclonal antibody that recognizes TAR DNA-binding protein 43 (gene TARDBP) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 44.7 kDa, and the supplier reports an observed band at about 45 kDa.
TDP-43 (TARDBP) is a nuclear RNA-binding protein with two RRM domains that binds GU-rich sequences to regulate splicing, mRNA stability and transport. Cytoplasmic aggregates of TDP-43 are a pathological hallmark of most ALS cases and of frontotemporal lobar degeneration, making it a central protein in neurodegeneration research.
| Target | TAR DNA-binding protein 43 (gene TARDBP; UniProt Q13148, human) |
|---|---|
| Clone | 26T75 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 44.7 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:2000 |
|---|---|
| Immunohistochemistry | 1:100–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20–1:100 |
Samples with a confirmed band (WB): HeLa cells, mouse brain, rat brain.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human glioma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human TDP43.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places TAR DNA-binding protein 43 in the nucleus, cytoplasm and mitochondrion. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
