| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tudor domain-containing protein 3; TDRD3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human TDRD3, which shares 96.3% and 100% amino acid (aa) sequence identity with mouse and rat TDRD3, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets tudor domain-containing protein 3 (gene TDRD3) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 73.2 kDa, and the supplier reports an observed band at about 65–73 kDa.
Tudor domain-containing protein 3 (TDRD3) is a scaffold protein whose Tudor domain recognizes asymmetrically dimethylated arginine. In the nucleus it reads active histone marks and acts as a transcriptional coactivator, and in the cytoplasm it joins stress granules and helps regulate translation.
| Target | Tudor domain-containing protein 3 (gene TDRD3; UniProt Q9H7E2, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 73.2 kDa |
| Observed MW | 65–73 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL |
Samples with a confirmed band (WB): human placenta tissue, human HL-60 cells, human HeLa cells, human PC-3 cells, human HEK293 cells, human A549 cells, human A431 cells, rat brain tissue, rat liver tissue, mouse brain tissue, mouse lung tissue, mouse liver tissue, mouse NIH3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue, mouse small intestine tissue, rat small intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): human intestinal cancer tissue.
Immunogen
Synthetic peptide from the N-terminal region of human TDRD3. Sequence identity with the mouse and rat orthologs: 96.3% and 100%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tudor domain-containing protein 3 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
