| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tyrosine-protein kinase Tec; TEC; PSCTK4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Tec, which shares 97.5% amino acid (aa) sequence identity with both mouse and rat Tec. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects tyrosine-protein kinase Tec in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 73.6 kDa, and the supplier reports an observed band at about 74 kDa.
TEC is the founding member of the Tec family of non-receptor tyrosine kinases, which also includes BTK and ITK. It acts downstream of antigen, cytokine and costimulatory receptors in T cells and B cells, largely overlapping with ITK and BTK, and it is studied in lymphocyte signaling and kinase inhibitor research.
| Target | Tyrosine-protein kinase Tec (gene TEC; UniProt P42680, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 73.6 kDa |
| Observed MW | 74 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|
Samples with a confirmed band (WB): human HEL cells, human THP-1 cells, rat kidney tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human tec. Sequence identity with the mouse and rat orthologs: 97.5%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places tyrosine-protein kinase Tec in the cytoplasm and cell membrane. Tissue expression noted by UniProt (human): Expressed in a wide range of cells, including hematopoietic cell lines like myeloid, B-, and T-cell lineages. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
