| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Axin-2;Axin-like protein;Axil;Axis inhibition protein 2;Conductin;AXIN2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human TFE3 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-TFE3 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AFHC-20; isotype Rabbit IgG; reactivity: Human. Reported application contexts include IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-TFE3 Monoclonal Antibody catalog # M01791. Tested in IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: TFE3 (Axin-2).
- Antibody format: Monoclonal; clone AFHC-20; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
TFE3 (protein: Lysosome-associated membrane glycoprotein 2 (Lamp2)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta- catenin and APC by GSK3B (By similarity). . Reported cellular localization context: Cytoplasm. Tissue expression notes (as provided): Expressed in brain and lymphoblast.
Research relevance and current trends
- Research context keywords from the source record include: Adaptive Immunity,Cancer Susceptibility,Domain Families,Epigenetics and Nuclear Signaling,Hlh/Leucine Zipper,Immunology,Non-CD,Proto-Oncogenes,T Cells,Transcription.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate TFE3 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect TFE3 in FFPE tissue sections by immunohistochemistry, Localize TFE3 by immunofluorescence/immunocytochemistry in cultured cells, Quantify TFE3-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 40 kDa; calculated MW: 93558 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 40 kDa
- Cellular localization (provided): Cytoplasm.
- Tissue details (provided): Expressed in brain and lymphoblast.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
