| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor E3; Class E basic helix-loop-helix protein 33; bHLHe33; TFE3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TFE3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFHC-20 is a rabbit monoclonal antibody that recognizes transcription factor E3 (gene TFE3) in human samples and is validated for IHC, ICC/IF and flow cytometry. The predicted molecular weight is 61.5 kDa, and the supplier reports an observed band at about 70 kDa.
TFE3 is a basic helix-loop-helix leucine zipper transcription factor of the MiT/TFE family, alongside TFEB and MITF. Under nutrient-rich conditions mTORC1 phosphorylates and inactivates it, while starvation or lysosomal stress lets it enter the nucleus and switch on lysosomal and autophagy genes; TFE3 gene fusions define certain renal cell carcinomas.
| Target | Transcription factor E3 (gene TFE3; UniProt P19532, human) |
|---|---|
| Clone | AFHC-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 61.5 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Immunohistochemistry | 1:50–1:200 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:100 |
| Flow cytometry | 1:50 |
Samples with confirmed staining (IHC): human bladder tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human TFE3.
Reactivity Notes
The supplier lists reactivity with human. UniProt places transcription factor E3 in the cytoplasm, nucleus and lysosome membrane. Tissue expression noted by UniProt (human): Ubiquitous in fetal and adult tissues.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
