| Field | Specification |
|---|---|
| Target | |
| Alternative names | Trefoil factor 3; Intestinal trefoil factor; rITF; Polypeptide P1.B; rP1.B; Tff3; Itf; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived rat TFF3 recombinant protein (Position: Q23-F81). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects trefoil factor 3 (gene Tff3) in mouse and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 8.9 kDa, and the supplier reports an observed band at about 12 kDa.
Trefoil factor 3 (TFF3, also called intestinal trefoil factor) is a small secreted peptide made mainly by goblet cells of the intestine. It supports maintenance and repair of the intestinal lining by promoting epithelial cell migration over wounds, and it is studied in gut barrier function and cancer.
| Target | Trefoil factor 3 (gene Tff3; UniProt Q03191, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 8.9 kDa |
| Observed MW | 12 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat gaster tissue, rat small intestine tissue, mouse small intestine tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse gaster tissue, mouse small intestine tissue, rat gaster tissue, rat small intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant rat TFF3 fragment (Gln23–Phe81), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places trefoil factor 3 in the cytoplasm. Tissue expression noted by UniProt (rat): Expressed in goblet cells of the intestines, and colon, in paraventricular hypothalamus and supraoptic nuclei. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
JF Wu et al. (2014) Expression and localization of trefoil factor family genes in rat submandibular glands. Biotechnic & Histochemistry. 10.3109/10520295.2014.885565
