| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Trans-Golgi network integral membrane protein 2;TGN38 homolog;TGN46;TGN48;Trans-Golgi network protein TGN51;TGOLN2;TGN46, TGN51; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human TGN46 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-TGOLN2 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AOHO-20; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, IP (as provided in the source record). Boster Bio Anti-TGN46 Rabbit Monoclonal Antibody catalog # M06591. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Key elements and design rationale
- Target: TGOLN2 (Trans-Golgi network integral membrane protein 2).
- Antibody format: Monoclonal; clone AOHO-20; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
TGOLN2 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): May be involved in regulating membrane traffic to and from trans-Golgi network. Reported cellular localization context: Cell membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane; Single- pass type I membrane protein. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes. Tissue expression notes (as provided): Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.
Research relevance and current trends
- Research context keywords from the source record include: Organelles,Subcellular Markers,Tags & Cell Markers.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate TGOLN2 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect TGOLN2 expression by Western blot in cell or tissue lysates, Detect TGOLN2 in FFPE tissue sections by immunohistochemistry, Enrich TGOLN2 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 35 kDa; calculated MW: 51113 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 35 kDa
- Cellular localization (provided): Cell membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane; Single- pass type I membrane protein. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes.
- Tissue details (provided): Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
