Anti-TGN46 Rabbit Monoclonal Antibody

SKU:BHA21008197
—
Suppliers
Boster Bio
Boster Bio
Details Products
Overview
Click light‑blue chips for details
Anti-TGOLN2 antibody (Rabbit monoclonal, clone AOHO-20, Rabbit IgG). Validated for Western blot, immunohistochemistry and immunoprecipitation. Reactivity: Human. Recognizes trans-Golgi network integral membrane protein 2. Observed band in Western blot: about 90 kDa.
Target TGOLN2
clone number AOHO-20
Host Rabbit
Reactivity Human
Isotype Rabbit IgG
Application(s) WB, IHC, IP
Options selector
Catalog no. Size Conjugation
M06591 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB,IHC,IP
      Application details: WB 1:500-2000<br>IHC 1:50-200<br>IP 1:20<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target TGOLN2
Alternative names Trans-Golgi network integral membrane protein 2;TGN38 homolog;TGN46;TGN48;Trans-Golgi network protein TGN51;TGOLN2;TGN46, TGN51;
Gene ID 10618
UniProt # O43493
Host Rabbit
Clone AOHO-20
Clonality
  • Monoclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
Immunogen A synthesized peptide derived from human TGN46
Molecular weight 45.9 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Cell membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane; Single- pass type I membrane protein. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M06591
Main SKU BHA21008197
Rabbit Monoclonal

Product Overview

Clone AOHO-20 is a rabbit monoclonal antibody that recognizes trans-Golgi network integral membrane protein 2 (gene TGOLN2) in human samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 45.9 kDa, and the supplier reports an observed band at about 90 kDa.

TGN46 (TGOLN2, also known as TGN38 in rodents) is a type I integral membrane protein that cycles between the trans-Golgi network and the plasma membrane. It is thought to help regulate membrane traffic leaving and returning to the trans-Golgi network and is widely used as a marker of this compartment in cell biology studies.

Target Trans-Golgi network integral membrane protein 2 (gene TGOLN2; UniProt O43493, human)
Clone AOHO-20
Host / Clonality / Isotype Rabbit / Monoclonal / Rabbit IgG
Reactivity Human
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 45.9 kDa
Observed MW 90 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunohistochemistry 1:50–1:200
Immunoprecipitation 1:20

Immunogen

Synthetic peptide based on the sequence of human TGN46.

Reactivity Notes

The supplier lists reactivity with human. UniProt places trans-Golgi network integral membrane protein 2 in the cell membrane. Tissue expression noted by UniProt (human): Isoform TGN46 is widely expressed.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunohistochemistry 1:50–1:200; Immunoprecipitation 1:20. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.Is a carrier-free (BSA-free) version available, for example for conjugation?
A.Yes. A carrier-free formulation without BSA, stabilized with trehalose and/or glycerol, can be prepared on request and is compatible with common conjugation chemistries. Please note this when ordering or contact info@biohippo.com. For storage at −20 °C, keep a cryoprotectant such as glycerol in the buffer rather than PBS alone.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 45.9 kDa; observed band at about 90 kDa. A difference from the calculated size is common and can reflect post-translational modification, processing or isoform differences.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human TGN46.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Get a Quote

Please use this form for bulk quantity requests or customized products.

Contact Information

Product Information

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today