| Field | Specification |
|---|---|
| Target | |
| Alternative names | Thy-1 membrane glycoprotein;CDw90;Thy-1 antigen;CD90;THY1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human THY1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOFC-20 is a rabbit monoclonal antibody that recognizes thy-1 membrane glycoprotein (gene THY1) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 17.9 kDa, and the supplier reports an observed band at about 25 kDa.
Thy-1 (THY1, CD90) is a small GPI-anchored glycoprotein of the immunoglobulin superfamily found on the surface of neurons, thymocytes, T cells in mice, fibroblasts and some stem cells. It takes part in cell–cell and cell–matrix interactions, including in the nervous system. CD90 is widely used as a marker for T cells in mice, mesenchymal stromal cells and hematopoietic stem cells.
| Target | Thy-1 membrane glycoprotein (gene THY1; UniProt P04216, human) |
|---|---|
| Clone | AOFC-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 17.9 kDa |
| Observed MW | 25 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human fetal brain.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): U87-MG cells.
Immunogen
Synthetic peptide based on the sequence of human THY1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places thy-1 membrane glycoprotein in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liu Jun et al. (2018) Pre-vascularization in fibrin Gel/PLGA microsphere scaffolds designed for bone regeneration. NPG Asia Materials. 10.1038/s41427-018-0076-8
Zhang Qing et al. (2018) CD90 determined two subpopulations of glioma-associated mesenchymal stem cells with different roles in tumour progression. Cell Death & Disease. 10.1038/s41419-018-1140-6
