| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Thymidine kinase, cytosolic;2.7.1.21;TK1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human TK1 recombinant protein (Position: S2-N234). Human TK1 shares 87.1% and 91.7% amino acid (aa) sequence identity with mouse and rat TK1, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of TK1 (Thymidine kinase, cytosolic) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Thymidine Kinase 1/TK1 Antibody Picoband® catalog # PB9832. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human TK1 recombinant protein (Position: S2-N234). Human TK1 shares 87.1% and 91.7% amino acid (aa) sequence identity with mouse and rat TK1, respectively. (reported region: S2-N234).
- Molecular weight context: reported MW: 25 kDa; calculated MW: 25469 MW
- Reactivity: Human
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Thymidine kinase, cytosolic; Thymidine kinase, cytosolic. Thymidine kinase 1, soluble (gene name TK1), is a human thymidine kinase. Thymidine kinase catalyzes the phosphorylation of thymidine to deoxythymidine monophosphate. This gene is mapped to 17q23.2-q25.3. It is estimated that the maximal size of the TK1 gene is 14 kb and its minimal size between 4 and 5 kb. The gene contains many noncoding inserts and numerous Alu sequences. There are two forms of this protein, which have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells. Functional note: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. Reported localization: Cytoplasm. Expression/tissue context: Found in all tissues examined, most abundant in placenta and least abundant in brain and heart.
Research relevance and current trends
- DNA/RNA: Researchers commonly examine how TK1 (Thymidine kinase, cytosolic) relates to this theme using model systems and orthogonal readouts.
- DNA Synthesis: Researchers commonly examine how TK1 (Thymidine kinase, cytosolic) relates to this theme using model systems and orthogonal readouts.
- Epigenetics and Nuclear Signaling: Researchers commonly examine how TK1 (Thymidine kinase, cytosolic) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative TK1 (Thymidine kinase, cytosolic) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
