| Field | Specification |
|---|---|
| Target | |
| Alternative names | T-lymphoma invasion and metastasis-inducing protein 1;TIAM-1;TIAM1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human TIAM1, different from the related mouse sequence by five amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects rho guanine nucleotide exchange factor TIAM1 in human samples and is validated for Western blot. The predicted molecular weight is 177.5 kDa, and the supplier reports an observed band at about 178 kDa.
TIAM1 is a guanine nucleotide exchange factor that activates Rho-family GTPases, mainly RAC1 and to a lesser degree CDC42 and RHOA. By turning on RAC1, it links extracellular signals to actin cytoskeleton remodeling, cell adhesion, polarity and migration. TIAM1 is studied in cell motility, neuronal development and tumor invasion.
| Target | Rho guanine nucleotide exchange factor TIAM1 (gene TIAM1; UniProt Q13009, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 177.5 kDa |
| Observed MW | 178 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human TIAM1. It differs from the mouse sequence at five residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt places rho guanine nucleotide exchange factor TIAM1 in the cell junction and cell membrane. Tissue expression noted by UniProt (human): Found in virtually all analyzed tumor cell lines including B- and T-lymphomas, neuroblastomas, melanomas and carcinomas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
