| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitochondrial import inner membrane translocase subunit Tim17-A; Inner membrane preprotein translocase Tim17a; TIMM17A; MIMT17; TIM17; TIM17A; TIMM17 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human TIMM17A, which shares 88.2% and 82.4% amino acid (aa) sequence identity with mouse and rat TIMM17A, respectively. |
| Molecular weight | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects mitochondrial import inner membrane translocase subunit Tim17-A (gene TIMM17A) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 18.0 kDa, and the supplier reports an observed band at about 18 kDa.
TIM17A (TIMM17A) is a core subunit of the TIM23 translocase in the mitochondrial inner membrane. TIM23 imports proteins carrying N-terminal presequences into the inner membrane and matrix. TIMM17A is studied in mitochondrial protein import and the regulation of mitochondrial biogenesis under stress.
| Target | Mitochondrial import inner membrane translocase subunit Tim17-A (gene TIMM17A; UniProt Q99595, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 18.0 kDa |
| Observed MW | 18 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| IHC (frozen sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
Samples with a confirmed band (WB): rat heart tissue, rat lung tissue, rat liver tissue, rat brain tissue, mouse heart tissue, mouse lung tissue, mouse liver tissue, mouse brain tissue, mouse Neuro-2a cells, human placenta tissue, human K562 cells, human T-47D cells, human Caco-2 cells, human HeLa cells, human THP-1 cells, human A431 cells, human A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue, human lung cancer tissue, human mammary cancer tissue, rat lung tissue, mouse lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): HeLa cells, A549 cells.
Samples with a confirmed signal (flow cytometry): A431 cells.
Immunogen
Synthetic peptide from the middle region of human TIMM17A. Sequence identity with the mouse and rat orthologs: 88.2% and 82.4%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mitochondrial import inner membrane translocase subunit Tim17-A in the mitochondrion inner membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
