| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Heat shock 70 kDa protein 1A ;Heat shock 70 kDa protein 1;HSP70-1 ;HSP70.1;HSPA1A;HSPA1, HSX70; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human TIRAP |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-TIRAP antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 23T78; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB (as provided in the source record). Boster Bio Anti-TIRAP Rabbit Monoclonal Antibody catalog # M00950-1. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: TIRAP (Heat shock 70 kDa protein 1A).
- Antibody format: Monoclonal; clone 23T78; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
TIRAP (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation (PubMed:23973223). . Reported cellular localization context: Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Tissue expression notes (as provided): Expressed in the excretory system, thyroid gland and Wilms tumors.
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Chaperones,Heat Shock Proteins,Protein Trafficking,Signal Transduction,Tumor Biomarkers.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
Workflow ideas (metafield): Validate TIRAP antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect TIRAP expression by Western blot in cell or tissue lysates, Compare relative TIRAP levels across experimental conditions (dose/time-course) using antibody-based readouts
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 28 kDa; calculated MW: 70052 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 28 kDa
- Cellular localization (provided): Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Tissue details (provided): Expressed in the excretory system, thyroid gland and Wilms tumors.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
