| Field | Specification |
|---|---|
| Target | |
| Alternative names | Toll/interleukin-1 receptor domain-containing adapter protein; TIR domain-containing adapter protein; Adaptor protein Wyatt; MyD88 adapter-like protein; MyD88-2; TIRAP; MAL |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human TIRAP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23T78 is a rabbit monoclonal antibody that recognizes toll/interleukin-1 receptor domain-containing adapter protein (gene TIRAP) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 23.9 kDa, and the supplier reports an observed band at about 28 kDa.
TIRAP (also called Mal) is a TIR-domain adaptor that acts as a bridge between Toll-like receptors TLR2 and TLR4 and the adaptor MyD88. This recruitment leads through IRAK kinases and TRAF6 to NF-κB and MAP kinase activation and inflammatory cytokine release. TIRAP is studied in innate immunity, bacterial infection and inflammation.
| Target | Toll/interleukin-1 receptor domain-containing adapter protein (gene TIRAP; UniProt P58753, human) |
|---|---|
| Clone | 23T78 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 23.9 kDa |
| Observed MW | 28 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): rat spleen tissue, rat thymus tissue, mouse spleen tissue, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human TIRAP.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places toll/interleukin-1 receptor domain-containing adapter protein in the cytoplasm, cell membrane and membrane. Tissue expression noted by UniProt (human): Highly expressed in liver, kidney, spleen, skeletal muscle and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Fei Wang et al. (2020) HMGB1 promoted P-glycoprotein at the blood-brain barrier in MCAO rats via TLR4/NF-κB signaling pathway. European Journal of Pharmacology. 10.1016/j.ejphar.2020.173189
