| Field | Specification |
|---|---|
| Target | |
| Alternative names | Toll-like receptor 2;Toll/interleukin-1 receptor-like protein 4;CD282;TLR2;TIL4; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human TLR2 recombinant protein (Position: H610-S784). Human TLR2 shares 87.4% amino acid (aa) sequence identity with mouse TLR2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes toll-like receptor 2 (gene TLR2) in human samples and is validated for Western blot. The predicted molecular weight is 89.8 kDa, and the supplier reports an observed band at about 105 kDa.
Toll-like receptor 2 (TLR2) is a cell surface pattern recognition receptor that pairs with TLR1 or TLR6 to recognize bacterial lipoproteins, lipopeptides and other cell wall components. Through MyD88 and TRAF6 it activates NF-κB and triggers inflammatory cytokine release. TLR2 is widely studied in innate immunity, bacterial and fungal infection, and chronic inflammation.
| Target | Toll-like receptor 2 (gene TLR2; UniProt O60603, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 89.8 kDa |
| Observed MW | 105 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human THP-1 (-LPS) cells, human THP-1 (+LPS) cells, human Hacat cells, human PC-3 cells, human Caco-2 cells, human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human TLR2 fragment (His610–Ser784), expressed in E. coli. Sequence identity with the mouse ortholog: 87.4%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places toll-like receptor 2 in the membrane, cytoplasmic vesicle and membrane raft. Tissue expression noted by UniProt (human): Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Lang Bai et al. (2017) Effect of lentivirus-mediated gene silencing, targeting toll-like receptor 2, on corneal allograft transplantation in rats. Molecular Immunology. 10.1016/j.molimm.2017.08.022
Long Yue et al. (2016) Bletilla striata polysaccharide inhibits angiotensin II-induced ROS and inflammation via NOX4 and TLR2 pathways. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2016.05.002
Tong Xing et al. (2020) Enhanced cytokine expression and upregulation of inflammatory signaling pathways in broiler chickens affected by wooden breast myopathy. Journal of the Science of Food and Agriculture. 10.1002/jsfa.10641
Liu Ji Ping et al. (2015) Shikonin inhibits the cell viability, adhesion, invasion and migration of the human gastric cancer cell line MGC-803 via the Toll-like receptor 2/nuclear factor-kappa B pathway. Journal of Pharmacy and Pharmacology. 10.1111/jphp.12402
Dai Jun et al. (2013) A novel DNA vaccine expressing the Ag85A-HA2 fusion protein provides protection against influenza A virus and Staphylococcus aureus. Virology Journal. 10.1186/1743-422X-10-40
Lisi Hu et al. (2016) Expression of TLR2/4 on Epididymal Spermatozoa of the Chinese Soft-Shelled Turtle Pelodiscus sinensis During the Hibernation Season. Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology. 10.1002/ar.23463
