| Field | Specification |
|---|---|
| Target | |
| Alternative names | Toll-like receptor 2;Toll/interleukin-1 receptor-like protein 4;CD282;TLR2;TIL4; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Tlr2 recombinant protein (Position: K20-K683). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets toll-like receptor 2 (gene Tlr2) in mouse samples and is validated for Western blot and ELISA. The predicted molecular weight is 89.4 kDa, and the supplier reports an observed band at about 89 kDa.
Toll-like receptor 2 (TLR2) is a cell surface pattern recognition receptor that pairs with TLR1 or TLR6 to recognize bacterial lipoproteins, lipopeptides and other cell wall components. Through MyD88 and TRAF6 it activates NF-κB and triggers inflammatory cytokine release. TLR2 is widely studied in innate immunity, bacterial and fungal infection, and chronic inflammation.
| Target | Toll-like receptor 2 (gene Tlr2; UniProt Q9QUN7, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 89.4 kDa |
| Observed MW | 89 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant mouse Tlr2 fragment (Lys20–Lys683), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt places toll-like receptor 2 in the cell membrane, cytoplasmic vesicle and membrane raft. Tissue expression noted by UniProt (mouse): Detected in a macrophage cell line, smooth muscle, lung, spleen, thymus, brain and adipose tissue. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
