| Field | Specification |
|---|---|
| Target | |
| Alternative names | Toll-like receptor 4; CD antigen CD284; Tlr4; Lps |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Tlr4 recombinant protein (Position: N26-E827). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes toll-like receptor 4 (gene Tlr4) in mouse samples and is validated for Western blot and ELISA. The predicted molecular weight is 95.5 kDa, and the supplier reports an observed band at about 96 kDa.
Toll-like receptor 4 (TLR4) is the receptor for bacterial lipopolysaccharide (LPS), working with the co-receptor MD-2 (LY96) and CD14. It signals from the cell surface through MyD88 and, after internalization, through TRIF, activating NF-κB, IRF3 and inflammatory cytokine production. TLR4 is central to research on sepsis, endotoxin responses and sterile inflammation.
| Target | Toll-like receptor 4 (gene Tlr4; UniProt Q9QUK6, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 95.5 kDa |
| Observed MW | 96 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.5–1 µg/mL (Mouse) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant mouse Tlr4 fragment (Asn26–Glu827), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt places toll-like receptor 4 in the cell membrane, early endosome and cell projection. Tissue expression noted by UniProt (mouse): Expressed in macrophages (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ying Li et al. (2025) Ginsenosides Modulate Immunity via TLR4/MyD88/NF-κB Pathway and Gut Microbiota. Phytomedicine. 10.1016/j.phymed.2025.156763
Zhaowei Cai et al. (2023) Dysregulation of Ceramide Metabolism Is Linked to Iron Deposition and Activation of Related Pathways in the Aorta of Atherosclerotic Miniature Pigs. Antioxidants. 10.3390/antiox13010004
Lei Zhang et al. (2026) Effect of Puerarin on Chronic Alcoholic Encephalopathy by Modulating the “Microbiota-Gut-Brain Axis” Lipopolysaccharides/Toll-Like Receptors 4/Nuclear Factor Kappa-B Inflammatory Pathway. Phytotherapy Research. 10.1002/ptr.70197
Chuang Liu et al. (2022) An Acidic Polysaccharide with Anti-Inflammatory Effects from Blackened Jujube: Conformation and Rheological Properties. Foods. 10.3390/foods11162488
Tong Liu et al. (2016) Toll-like receptor 4 contributes to chronic itch, alloknesis and spinal astrocyte activation in male mice. Pain. 10.1097/j.pain.0000000000000439
Jianing Tan et al. (2023) Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration. International Journal of Molecular Medicine. 10.3892/ijmm.2023.5307
Hong-Xin Cui et al. (2018) Hypoglycemic Mechanism of the Berberine Organic Acid Salt under the Synergistic Effect of Intestinal Flora and Oxidative Stress. Oxidative Medicine and Cellular Longevity. 10.1155/2018/8930374
Chuanbo Sun et al. (2024) Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway. Journal of Ethnopharmacology. 10.1016/j.jep.2024.118067
Chengquan Han et al. (2025) Integrated network pharmacology, molecular docking and in vivo experiments to elucidate the extenuative mechanisms of ginseng polysaccharide against Toxoplasma gondii-induced testicular toxicity. International Immunopharmacology. 10.1016/j.intimp.2025.114147
Ying Li et al. (2023) UHPLC-MS-Based Metabolomics Reveal the Potential Mechanism of Armillaria mellea Acid Polysaccharide in and Its Effects on Cyclophosphamide-Induced Immunosuppressed Mice. Molecules. 10.3390/molecules28247944
